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rabbit
Rabbit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+asic3/Anti-ASIC3+Antibody/pmc12241711-116-9-12
Average 93 stars, based on 33 article reviews
Rabbit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+asic3/Anti-ASIC3+Antibody/pmc12241711-116-9-12
Average 93 stars, based on 33 article reviews
rabbit - by Bioz Stars,
2026-10
93/100 stars
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Membrane:Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS–T). .. The membrane was incubated with primary antibody in blocking solution overnight 4 °C with gentle rocking, washed in TBS–T, and probed with HRP-conjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), anti-ASIC2a (#ASC-012, Alomone labs), Article Title: NGF contributes to activities of acid‐sensing ion channels in dorsal root ganglion neurons of male rats with experimental peripheral artery disease Article Snippet: .. After blocking with 5% non‐fat milk in 0.1% Tween–TBS buffer for 1 h, the membrane was respectively incubated with rabbit anti‐ASIC1a (1:500, Alomone Lab Cat #ASC‐014), Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons. Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS–T). .. The membrane was incubated with primary antibody in blocking solution overnight 4 °C with gentle rocking, washed in TBS–T, and probed with HRPconjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), anti-ASIC2a (#ASC-012, Alomone labs), Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS– T). .. The membrane was incubated with primary antibody in blocking solution overnight 4°C with gentle rocking, washed in TBS–T, and probed with HRP-conjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), antiASIC2a (#ASC-012, Alomone labs), Incubation:Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS–T). .. The membrane was incubated with primary antibody in blocking solution overnight 4 °C with gentle rocking, washed in TBS–T, and probed with HRP-conjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), anti-ASIC2a (#ASC-012, Alomone labs), Article Title: Contribution of retrotrapezoid nucleus neurons to CO 2 ‐amplified cardiorespiratory activity in spontaneously hypertensive rats Article Snippet: The respiratory regulation of cardiovascular activity is essential for maintaining an efficient ventilation and perfusion ratio.. Activation of central respiratory chemoreceptors not only elicits a ventilatory response but also regulates sympathetic nerve activity and arterial blood pressure (ABP).. The retrotrapezoid nucleus (RTN) is the most completely characterized cluster of central respiratory chemoreceptors. Article Title: NGF contributes to activities of acid‐sensing ion channels in dorsal root ganglion neurons of male rats with experimental peripheral artery disease Article Snippet: .. After blocking with 5% non‐fat milk in 0.1% Tween–TBS buffer for 1 h, the membrane was respectively incubated with rabbit anti‐ASIC1a (1:500, Alomone Lab Cat #ASC‐014), Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons. Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS–T). .. The membrane was incubated with primary antibody in blocking solution overnight 4 °C with gentle rocking, washed in TBS–T, and probed with HRPconjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), anti-ASIC2a (#ASC-012, Alomone labs), Article Title: Contribution of retrotrapezoid nucleus neurons to CO 2 ‐amplified cardiorespiratory activity in spontaneously hypertensive rats Article Snippet: The membranes were blocked with bovine serum albumin and incubated at 4 °C overnight with primary antibodies that have been validated before (Gonczi et al. 2006; Huang et al. 2010): anti-Kv12.1 (dilution 1:200, Cat# APC-113, RRID:AB_2039939, Alomone labs, Israel), anti-acid-sensing ion channel 1 (ASIC1, dilution 1:200, Cat# ASC-014, RRID:AB_2039697, Alomone labs, Israel), anti-ASIC2 (dilution 1:200, Cat# ASC-012, RRID:AB_2039699, Alomone labs, Israel), anti-ASIC3 (dilution 1:200, Cat# ASC-018, RRID:AB_2039701, Alomone labs, Israel), anti-TASK-2 (dilution 1:200, Cat# APC-037, RRID:AB_2040134, Alomone labs, Israel) and anti-GAPDH (dilution 1:3000, Cat# EM1101, HuaAn Biotechnology, China, RRID AB_2811078). Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS– T). .. The membrane was incubated with primary antibody in blocking solution overnight 4°C with gentle rocking, washed in TBS–T, and probed with HRP-conjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), antiASIC2a (#ASC-012, Alomone labs), Blocking Assay:Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS–T). .. The membrane was incubated with primary antibody in blocking solution overnight 4 °C with gentle rocking, washed in TBS–T, and probed with HRP-conjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), anti-ASIC2a (#ASC-012, Alomone labs), Article Title: NGF contributes to activities of acid‐sensing ion channels in dorsal root ganglion neurons of male rats with experimental peripheral artery disease Article Snippet: .. After blocking with 5% non‐fat milk in 0.1% Tween–TBS buffer for 1 h, the membrane was respectively incubated with rabbit anti‐ASIC1a (1:500, Alomone Lab Cat #ASC‐014), Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons. Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS–T). .. The membrane was incubated with primary antibody in blocking solution overnight 4 °C with gentle rocking, washed in TBS–T, and probed with HRPconjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), anti-ASIC2a (#ASC-012, Alomone labs), Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS– T). .. The membrane was incubated with primary antibody in blocking solution overnight 4°C with gentle rocking, washed in TBS–T, and probed with HRP-conjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), antiASIC2a (#ASC-012, Alomone labs), Gentle:Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS–T). .. The membrane was incubated with primary antibody in blocking solution overnight 4 °C with gentle rocking, washed in TBS–T, and probed with HRP-conjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), anti-ASIC2a (#ASC-012, Alomone labs), Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons. Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS–T). .. The membrane was incubated with primary antibody in blocking solution overnight 4 °C with gentle rocking, washed in TBS–T, and probed with HRPconjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), anti-ASIC2a (#ASC-012, Alomone labs), Article Title: Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons Article Snippet: The proteins were then transferred to a 0.45 μm pore size PVDF membrane (#10600023, Cytiva), which was blocked by immersion in blocking solution containing 5% fat-free dry milk powder in TBS with 0.05% Tween (Sigma Aldrich), pH 7.5 (TBS– T). .. The membrane was incubated with primary antibody in blocking solution overnight 4°C with gentle rocking, washed in TBS–T, and probed with HRP-conjugated secondary antibodies (1:10.000 in blocking solution) for 1 h. The following primary antibodies were used: anti-ASIC1 (#833501, Biolegend), antiASIC2a (#ASC-012, Alomone labs), |
![Generation of a virtual 3D model of the human ASIC3 channel. ( a ) AlphaFold model of human ASIC3 represented as cartoons colored by model quality (from low quality pLDDT = 50 in blue, to high quality pLDDT = 100 in red); inset: close up view of the extracellular domain of ASIC3, highlighting with spheres amino acids Glu78 and Glu421, known to be involved in GMQ binding . ( b ) Superposition of the two structural models (depicted as cartoon) selected after MD simulation using cluster analysis: 682 (68.2 ns) as blue and 812 (81.2 ns) as cyan and extent of the region explored during in silico docking analysis. ( c,d) Close-up views of the interaction between charged (yellow carbon atoms) and non-charged (orange carbons) GMQ with protein residues [sticks with carbon atoms colored in cyan (812) or blue (682)].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2124/pmc11372124/pmc11372124__41598_2024_71623_Fig2_HTML.jpg)
